منابع مشابه
Gene targeting: Things go better with Cre
New technologies are changing the way in which gene targeting experiments are being designed. It is now becoming possible to analyze gene function in defined tissues at specific times during the life of a mouse.
متن کاملGermline recombination by conditional gene targeting with Parvalbumin-Cre lines
Conditional gene targeting allows us to study gene function in specific tissues or cell types. This is commonly achieved by Cre DNA recombinase and its 34–base pair target sequences called loxP sites. Through the efforts of individual labs and largescale projects, a sizable collection of Cre mouse lines has been generated to express or delete specific genes in a wide range of cell types through...
متن کاملThe Cre/loxP system and gene targeting in the kidney.
The Cre/loxP and Flp/FRT systems mediate site-specific DNA recombination and are being increasingly utilized to study gene function in vivo. These systems allow targeted gene disruption in a single cell type in vivo, thereby permitting study of the physiological and pathophysiological impact of a given gene product derived from a particular cell type. In the kidney, the Cre/loxP system has been...
متن کاملInducible gene targeting in mice using the Cre/lox system.
Molecular techniques now allow the design of precise genetic modifications in the mouse. Not only can defined nucleotide changes be engineered into the genome of the mouse, but genetic switches can be designed to target expression or ablation of any gene (for which basic molecular information is available) to any tissue at any defined time. These strategies promise to contribute substantially t...
متن کاملGenomic targeting with purified Cre recombinase.
Purified Cre recombinase protein introduced directly into cultured mammalian cells by lipofection catalyzes both site-specific chromosomal integration of a co-transfected lox targeting vector and precise excision of genomic DNA flanked by directly repeated lox sites. This procedure eliminates the need to transfect cre expression plasmids to activate recombination at lox sites. We used this simp...
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ژورنال
عنوان ژورنال: Current Biology
سال: 1997
ISSN: 0960-9822
DOI: 10.1016/s0960-9822(06)00149-7